BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3659

Peptides:

Sequences

Affinity Values

VSGPSEL
LMGPSEL
NTGPSEL
LTGPSEL
FLGPSEL
GLGPSEL
VVGPSEL
GWGPSEL
HKGPWEL
YPSELGP
YPSELGR
NSGPSEL
MWGPNEL
GASELGK
QASELGN

[1.251±0.0806]
[1.8559±0.1059]
[1.619±0.0655]
[1.4224±0.0504]
[1.2661±0.0655]
[1.9365±0.0907]
[1.5938±0.0504]
[1.7652±0.0353]
[1.1301±0.0655]
[1.498±0.0403]
[1.1603±0.0907]
[1.0645±0.0554]
[0.8276±0.1059]
[0.999±0.0806]
[0.4748±0.0403]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (competitive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 30315830

Target Name:

Anti-Api m 1 IgG

Template Name:

Phospholipase A2, bvPLA2

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Absorbance was measured at 450 nm with a microtiter plate reader. A(450 nm) were reproduced from Figure E1A and shown.

Brief Description:

Bound phages were eluted from the target antibodies either with 0.1 mol/L glycine-HCl (pH 2.2) for 10 minutes followed by immediate neutralization with 1 mol Tris (pH 8.0) or competitively with Api m 1 at the final concentration of 12 mg/mL.

BiopanningDataSet Comments:

Curator:

Bowen Li

Curation Date:

2023-10-13

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