BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3625

Peptides:

Sequences
CAINSLSRKC(21%) CAKSMGDIVC(11%) CGRKQVESSC(10%) CRGKSAEGTC(5%)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (in vivo)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 33870243

Target Name:

Integrin alpha-3

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

fUSE5-based CX8C phage display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Phage was 10^9 TU of the phage library per mouse administered via the intratracheal route with 50 μL of PBS with a MicroSprayer® Aerosolizer coupled to a high-pressure syringe (Penn-Century) and a small animal laryngoscope (Penn-Century). The devices were used to administer air-free liquid aerosol directly into the trachea of animals deeply anesthetized with 1% isoflurane. The four rounds of selection were performed as described. In round one (R1), animals received 109 TU of the CX8C library via aerosol (see scheme in Figure 1B). After 60 min, phage particles were recovered from the bloodstream, amplified and pooled. In round two (R2), R1-pooled phage particles (i.e., inhaled phage sub-library 1) were administered and recovered 30 min post-administration. The subsequent R2-pooled phage particles (i.e., inhaled phage sub-library 2) were amplified for administration in round three (R3). After 10 min, the R3-pooled phage particles (i.e., inhaled phage sub-library 3) were recovered and processed for aerosol administration in the final round four (R4). After 5 min, phage particles were recovered from the bloodstream, amplified, and the corresponding peptide-encoding genomes were analyzed by DNA sequencing.

BiopanningDataSet Comments:

We screened a phage display random peptide library in vivo to select, identify, and validate a ligand (CAKSMGDIVC) that specifically targets and is internalized through its receptor, α3β1 integrin, on the surface of cells lining the lung airways and alveoli and mediates CAKSMGDIVC-displaying phage binding and systemic delivery without compromising lung homeostasis. As a proof-of-concept, we show that the pulmonary delivery of targeted CAKSMGDIVC-displaying phage particles in mice and non-human primates elicit a systemic and specific humoral response.

Curator:

Bowen Li

Curation Date:

2023-10-13

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