BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3573

Peptides:

Sequences
CTEWDYLTVC

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 33508757

Target Name:

Prostate specific antigen (PSA(-/lo)) human prostate cancer cell line LNCaP

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

fUSE55-based CX8C phage display library (CX8C)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Prostate cancer (PCa) contains both differentiated (PSA(+)) and undifferentiated (PSA(−/lo)) tumor cells. PSA was short for prostate-specific antigen. PSA(+) and PSA(-/lo) cells were mixed in equal proportions and incubated with CX8C phage display library. A measure of 1.0e9 of the phage display peptide library in 5 ml of 1 ×phosphate buffered saline (PBS) was added to the confluent cell monolayer and incubated for 2 h on a rocker platform at 4 °C. The cells were washed four times with PBS containing 0.2% Tween 20. Cells were subjected to fluorescence-activated cell sorting (FACS) for GFP(+) and GFP(−) cells. Sorted cells were lysed to release phage particles that were used to infect K91 bacteria. Individual bacterial colonies that emerged on the Tet/Kanamycin plates were isolated, amplified, and phage particles reisolated and used in 2–3 more similar cycles.

BiopanningDataSet Comments:

PSA(−/lo) PCa cell-specific targeting peptide (TAP1, CTEWDYLTVC) suppressed PCa cell growth both in vitro and in vivo and improved the drug sensitivities of anti-androgens and chemotherapeutic agents at least through shortening the length of telomere and reducing the expression of HOXB9 and TGF-β2.

Curator:

Bowen Li

Curation Date:

2023-10-13

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