BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3572

Peptides:

Sequences	Affinity Values
LYALPLSHLKSH(13) SPSSAYPRHGPD(12) DPLLFPGTSRQM(6)	[95.55%] [87.50%] [47.00%]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

2-4

Reference:

PMID: 37044252

Target Name:

Daphnia magna asexual egg

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Binding assay

Affinity Measurement Description:

Ten microliters of each phage was added to 200 μL of ER2738 culture grown to an OD600 of 0.7 and incubated at RT for 5 min. The cells with infected phages were transferred to a conical tube containing 5 mL of Top Agar at around 40 ◦C and poured directly onto an LB/IPTG/Xgal plate containing 1 mL of IPTG/Xgal stock in 25 mL of DMF. After standing for 1 h to solidify, the plates were incubated at 37 ◦C overnight. After counting the number of blue plaques on each plate, we calculated the pfu/mL value while considering the dilution factor of each phage. Attached % was calculated as: ((Input phage-Output phage)/Input phage) × 100.

Brief Description:

BiopanningDataSet Comments:

We identified a peptide, DEP1 (LYALPLSHLKSH), with the highest binding affinity to D. magna eggs. DEP1 did not affect zebrafish eggs, but it inhibited normal hatching and reproductive ability in D. magna eggs, and hindered growth in neonates before their first ecdysis. Morphological analysis revealed that DEP1 caused intestinal damage and tissue abnormalities. Our findings demonstrate that the whole cell-based phage display technique is successful in presenting antigens in their natural form, and that the DEP1 peptide can be applied to regulate the growth cycle of D. magna. These results have implications for the use of phage display in environmental research and the potential use of DEP1 for hazardous organisms in aquatic systems.

Curator:

Bowen Li

Curation Date:

2023-10-13

First |

Prev |

3572

| Next

| Last