BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3530

Peptides:

Sequences	Affinity Values
SGVYKVAYDWQH(5) SWFSDWDLELHA(5) SQDIRTWNGTRS(3) ETWLRWSEKYPT(2)	[NA] [56.40442] [NA] [66.06515]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 32892011

Target Name:

N-propyl paraben (PP)

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

The binding affinity and selectivity test of the selected peptide that has binding affinity to target was carried out using Dynabeads® M-270 Amine. Data (ppm) shown were reproduced from Figure 1b in the reference.

Brief Description:

The Ph.D.-12 Phage Display Peptide Library(New England BioLabs,Beverly, MA, USA) were used for affinity selection of specific peptides under three rounds of biopannning for the n-propyl paraben (PP)(≥99.0%) .

BiopanningDataSet Comments:

We successfully screened two peptides specific to PP, namely PP3; the results showed that the PP concentration reduction in PP3 was the highest at 39%, and the specificity was measured by the capture rate between target and control.

Curator:

Chunchao Pu

Curation Date:

2020-12-29

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