BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3529

Peptides:

Sequences	Affinity Values
EGGDSRH RLRAIFS VSRVAPC WLEVYLG	[0.137913±0.000046] [0.134717±0.000040] [0.129523±0.000046] [0.138723±0.000046]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 31344770

Target Name:

Pancreatic cancer cell line MIA PaCa-2

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Immunocytochemistry (ICC), enzyme-linked immunosorbent assay (ELISA), and fluorescence- activated cell sorter (FACS) were performed to examine the specific binding. Absorbance was determined at 450 nm. OD450 was reproduced from Fig. 1 in the reference.

Brief Description:

BiopanningDataSet Comments:

M5(WLEVYLG) bound to Ce6 showed a significantly lower cell survival rate than that of Ce6 alone in photodynamic therapy, which was observed consistently as a change in the tumor size and fluorescence intensity in MIA PaCa-2 cell-implanted animal models. The noble peptide, M5, binds specifically to the pancreatic cancer cell line, MIA PaCa-2. The M5 peptide has potential use in future optical diagnostic and therapeutic purposes.

Curator:

Chunchao Pu

Curation Date:

2020-12-29

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