BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3526

Peptides:

Sequences	Affinity Values
ANLNLWTDYIRW	[2.97436]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 32454443

Target Name:

Human colon cancer cell line HT29

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

The binding ability of the selected phage clones was evaluated on colon cancer cells (SW480, HT29, HCT116, LoVo, and DLD1) as well as on a normal cell line (CCD-18co) by cell phage ELISA. Absorbance was measured at 450 nm (OD450) using a plate reader. The binding affinities (relative OD values) showed more than two- to five-fold higher selectivity for colorectal cells than the negative controls. The binding affinity (relative fold) was reproduced from the Supplementary Figure 1 and shown.

Brief Description:

The Ph.D.-12 Phage Display Peptide Library(New England BioLabs, Beverly, MA) were used for affinity selection of specific peptides under three rounds of biopannning for the human colon cancer cells HT29.

BiopanningDataSet Comments:

The phage clone with the highest optical density (450 nm) compared to the negative control cells was selected. This peptide(ANLNLWTDYIRW) probe maintained binding affinity even after serum incubation. For therapeutic applications, this peptide probe was conjugated to hematoporphyrin, a photosensitizer, which showed a significantly enhanced cellular uptake and high photodynamic effect to kill tumor cells. As another application, we made a nanoparticle modified from the peptide probe. It efficiently delivered SN-38, an anticancer drug, into tumor cells, and its tumor-targeting ability was observed in vivo after intravenous injection to the same xenograft model.

Curator:

Chunchao Pu

Curation Date:

2020-12-29

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