BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3519

Peptides:

Sequences	Affinity Values
SPAQHTHERAHT(21) SPHLHTSSPWER(17) MKAHHSQLYPRH(12) NYPHPHLSNYHP(8) NAPKHAHRLPVH(2)	[13.07 ± 5.88] [217.97 ± 27.01] [1548.22 ± 835.51] [637.02 ± 815.98] [1282.66 ± 269.73]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 32828793

Target Name:

Amyloid beta 42, Aβ42

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Absorbance was measured at 450 nm (OD450) using a microplate reader (Molecular Devices). The Kd value (pM) was determined and shown.

Brief Description:

Two rounds of negative selection were performed for reducing non-specific phages, by adding the amplified phage to non-target-coated resin for 1 h. One was performed after the second round of positive selection, the other after the third round of positive selection. After centrifugation, non-bound phages in the supernatant were used for the following cycles.

BiopanningDataSet Comments:

The synthesized peptides were confirmed to have excellent binding affinity to Aβ aggregates, by immunohistochemical staining and western blotting using the brains of 3X transgenic (Tg) AD mice at different stages (5–7, 12–17 months old) of AD severity. In the present study, it was confirmed that newly developed amyloid-binding peptides could be used as novel probes for the detection of Aβ aggregates, which can be used for clinical diagnosis of AD in the future.

Curator:

Chunchao Pu

Curation Date:

2020-12-29

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