BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3518

Peptides:

Sequences	Affinity Values
GSAPLLTVDTSK(22) RVAPDFSCAYPY(18) SGVYKVAYDWQH(13) QSHDQNNYNQRS(7)	[12.45 ± 7.40] [12.62 ± 11.01] [35.80 ± 18.22] [NA]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 32828793

Target Name:

Amyloid beta 42, Aβ42

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Absorbance was measured at 450 nm (OD450) using a microplate reader (Molecular Devices). The Kd value (pM) was determined and shown.

Brief Description:

Negative selection was performed for reducing non-specific phages between the third and forth round of panning, adding the amplified phage to non-target coated plate for 1 h. Non-bound phages in the supernatant were used for the forth round of biopanning.

BiopanningDataSet Comments:

The synthesized peptides were confirmed to have excellent binding affinity to Aβ aggregates, by immunohistochemical staining and western blotting using the brains of 3X transgenic (Tg) AD mice at different stages (5–7, 12–17 months old) of AD severity. In the present study, it was confirmed that newly developed amyloid-binding peptides could be used as novel probes for the detection of Aβ aggregates, which can be used for clinical diagnosis of AD in the future.

Curator:

Chunchao Pu

Curation Date:

2020-12-29

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