BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3494

Peptides:

Sequences	Affinity Values
TMANEAPYPGTQ HWDPPYPGSGTQ TEAPYPGSSVTN SALIDAAYPGTQ SEAPYPGAGVYN TFEAPYPGTMLV STEAPYPGIAVQ	[1.41642] [1.32673] [1.20625] [1.14333] [1.1942] [1.12995] [1.31646]

Sequences Features:

Motif:

EAPYPG

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 31500272

Target Name:

Anti-σA monoclonal antibody 4E2

Template Name:

σA protein

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Reactivity of phage clones to MAb 4E2 was determined by enzyme-linked immunosorbent assay. Data (OD450) were reproduced from Figure 3a in the reference.

Brief Description:

Purified MAb 4E2 was selected for epitope mapping under three rounds of biopanning with the Ph.D-12 TM Phage Display Peptide Library Kit (New England BioLabs, Cambridge, MA, USA).

BiopanningDataSet Comments:

Sequencing of the phage clones with high OD values revealed the consensus sequence EAPYPG, which was identical to the 56EAPYPG61 (aa 217 to 223) of the DRV σA protein. Residues in epitope 56EAPYPG61 were completely homologous in DRV, GRV, ARV, and TRV.

Curator:

Chunchao Pu

Curation Date:

2020-12-01

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