BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3493

Peptides:

Sequences	Affinity Values
NHRKVSRHATHF(4) PRHGKKPTNKRK(3) TVDSASLLQSRT(1) WGFHWPVYPPSR(1) ADARPWWKSQGF(1) FPYPMNKQTNGT(1)	[0.08643] [0.79724] [0.08422] [0.07299] [0.08063] [0.08574]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (competitive panning)

Sequencing Platform:

Sangon Biotech, Shanghai, Chi

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 31823885

Target Name:

Lectin

Template Name:

α2,6-sialyllactose, 6'-SL

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Absorbance was determined at 415 nm in a microtiter plate reader (Tecan Infinite® M1000 Pro, Tecan, Switzerland). Data (OD415) shown were reproduced from Figure 1 in the reference.

Brief Description:

Bound phages were eluted using 100 μL of 1 M α2,6-sialyllactose (6′-SL) (Cat# 35890-39-2, Dextra Laboratories, Reading, England, UK) in each round of panning.

BiopanningDataSet Comments:

Mimetic peptide (PRHGKKPTNKRK), reverse peptide (KRKNTPKKGHRP) and scrambled peptide (RPGHKKTPKNKR) were tested for inhibition of 6′-SL binding to the lectin. Indeed, lectin binding to 6′-SL was inhibited by the mimetic peptide , but not by the reverse or scrambled peptides, showing that this peptide mimics 6′-SL. Functiolly, mimetic peptide, but not the reverse or scrambled peptides, increased viability and expression of neural cell adhesion molecule L1 in SK-N-SH human neuroblastoma cells, and promoted survival and neurite outgrowth of cultured mouse cerebellar granule neurons challenged by H2O2-induced oxidative stress. The combined results indicate that the 6′-SL mimetic peptide promotes neurol survival and neuritogenesis, thus raising hopes for the treatment of neurodegenerative diseases.

Curator:

Chunchao Pu

Curation Date:

2020-12-01

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