BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3456

Peptides:

Sequences	Affinity Values
CTTNPFSLC(6/12) CQAPHKPWC(2/12) CRLSMETVC(1/12) CPSSMRGTC(1/12) CDLLESERC(1/12) CKMWNGSGC(1/12)	[2913.0 ± 311.0] [NA] [NA] [NA] [NA] [NA]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 31173215

Target Name:

Bone mesenchymal stem cell (BMSC)

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-C7C phage display library (CX7C)

Affinity Measurement Method:

Flow Cytometry

Affinity Measurement Description:

The bone mesenchymal stem cell (BMSC) affinity properties of FITC-labeled peptides were analyzed quantitively by flow cytometry at a wavelength of 488 nm, using FlowJo v10 (Tree Star, Inc).

Brief Description:

BiopanningDataSet Comments:

A specific cyclic peptide for Sprague-Dawley rat bone mesenchymal stem cells (BMSCs), CTTNPFSLC (known as C7), was used, which was identified via phage display technology. Its high affinity for BMSCs was demonstrated using flow cytometry and fluorescence staining. Subsequently, the cyclic peptide was placed on β-tricalcium phosphate (β-TCP) scaffolds using absorption and freeze-drying processes. Adhesion, expansion and proliferation of BMSCs was investigated in vitro on the C7-treated β-TCP scaffolds and compared with pure β-TCP scaffolds. The results revealed that C7 had a promoting effect on the adhesion, expansion and proliferation of BMSCs on β-TCP scaffolds. Therefore, C7 may be effective in future tissue engineering therapy for osteonecrosis of the femoral head (ONFH).

Curator:

Lixu Jiang

Curation Date:

2019-08-16

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