BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3397

Peptides:

Sequences
GTGSQAS(4) GVEGHKP(3) QLAVAPS(2) MNSNIPI(2) TVNFKLY(2) ANPKNFS(1) FSGGGNH(1) ELWSLYD(1) NLQPPAY(1) RHFIKEL(1) NDLMNRA(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 30592604

Target Name:

Gold

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

The biopanning process was performed using the gold-patterned microfluidic chip with NEB Ph.D.-7 phage display peptide library kit. Ten microliters of phage solution (2e12 pfu mL−1) in 0.1 vol % PBST (PBS (pH 7.4) containing 0.1 vol % Tween 20) was loaded into the microfluidic biopanning platform at a flow rate of 1 μL min−1 at room temperature. Then PBST (0.1 vol % Tween 20 in the first round and 0.5 vol % Tween 20 from the second round) was introduced at 0.5 mL min−1 for 30 min to remove nonspecifically or weakly bound phages. The remaining high-affinity phages were eluted by injecting an elution buffer (0.2 M glycine-HCl (pH 2.2) with 1 mg mL−1 BSA) at 0.1 mL min−1 for 10 min, and the eluate was neutralized with 150 μL of 1 M Tris-HCl (pH 9.1). As the rounds progress, the proportion of high-aﬃnity bacteriophages increases and fewer bacteriophages are removed from the surface after the washing step. Thus, the number of eluted bacteriophages increases as the rounds continue.

BiopanningDataSet Comments:

Curator:

Ying Wang

Curation Date:

2019-05-16

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