BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3373

Peptides:

Sequences
KVWILPA(6/30) KVWVIPS(4/30)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 30521966

Target Name:

Virus-like particle (VLP) of orange-spotted grouper nervous necrosis virus (OGNNV)

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Briefly, 150 μL of the purified RBS (100 μg/mL) were coated with coating buffer (0.1 M NaHCO3, pH 8.6) in each well of the 96-well plate. After blocking with BSA (5 mg/mL) in coating buffer and washing 5 times with 0.1% TBST (50 mM Tris-HCl, 150 mM NaCl, 0.1% [v/v] Tween-20, pH 7.5), the well containing RBS was incubated with 100 μL of diluted library (5 × 1011 sequences) for 1 h at room temperature. After washed 10 times with TBST, bound phages were eluted with elution buffer (0.2 M glycine, 1 mg/mL BSA, pH 2.2) and neutralized with 1 M Tris (pH 9.1). Eluates were incubated with Escherichia coli ER2738 cells and amplified for 5 h at 37 °C. The amplified phages were collected, purified, and titrated for the next round of surface panning procedure.

BiopanningDataSet Comments:

Curator:

Zhongjie Tang

Curation Date:

2019-01-29

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