BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3372

Peptides:

Sequences

Affinity Values

CSGTGASYC(3)
CMSTGLSSC(2)
CNTGSPYEC(2)
CTASQNFYC(2)
CGSRSAQTC(1)
CGTKGSLNC(1)
CGYSSFNRC(1)
CHHPVANTC(1)
CHNETQKMC(1)
CKDTSRSAC(1)
CNAKHHPRC(1)
CNGRAVNYC(1)
CPGASVTYC(1)
CRAEGTSEC(1)
CRGATPMSC(1)
CSLATDQKC(1)
CSNNHSSMC(1)
CSTATPYKC(1)
CTKTDVHFC(1)
CTSVLNNTC(1)
CVPILEGTC(1)

[5.9 ± 1.7]
[4.0 ± 2.9]
[1.2 ± 0.0]
[10.4 ± 0.0]
[2.0 ± 1.1]
[17.8 ± 3.0]
[3.6 ± 1.2]
[1.1 ± 0.2]
[1.6 ± 0.6]
[1.2 ± 0.1]
[16.8 ± 6.5]
[0.9 ± 0.5]
[1.1 ± 1.0]
[1.0 ± 0.0]
[1.0 ± 0.7]
[2.1 ± 0.8]
[2.2 ± 1.0]
[2.4 ± 1.5]
[2.6 ± 2.0]
[2.4 ± 0.4]
[1.9 ± 0.8]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 29928986

Target Name:

Nickel ion

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-C7C phage display library (CX7C)

Affinity Measurement Method:

Binding assay

Affinity Measurement Description:

Pre-identified phage, derived from single clone amplification out of colonies of infected cells, were characterized concerning their ability to bind to individual components of fluorescent lamp powder and to quantify the selective binding behavior. Components tested were CeMgAl11O19:Tb3þ (CAT), Y2O3:Eu3þ (YOX), BaMgAl10O17:Eu2þ (BAM), LaPO4:Ce3þ,Tb3þ (LAP) and halophosphate (HP). Mineral preparation, phage production and binding studies were performed. Mineral dispersions were incubated together with the phage and subsequently repeatedly washed, resulting in the removal of weakly bound phage. Phage concentrations in input and eluate were determined and compared to the binding behavior of wild-type fd-tet phage (obtained by Dr. Jamie Scott). Binding efficiency was calculated using phage titer before and after binding assays in comparison to the wild-type phage.

Brief Description:

BiopanningDataSet Comments:

Biopanning was carried out on planar sol–gel surfaces. Sol-gels with cation exchange properties were fabricated using Dispex N40, polyacrylic acid or polystyrene sulfonate.29 colonies were examined for their peptide sequence.

Curator:

Zhongjie Tang

Curation Date:

2019-01-29

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