BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3358

Peptides:

Sequences	Affinity Values
TRWLVYFSRPYLVAT(8) PRHVFYRWFLSNPRI(4) IVRHLFLHVYPRVLM(1)	[4.4] [NT] [NT]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID:

Target Name:

Barley α-amylase 1

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

f3-15mer phage display library (X15)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

The phage clone was analyzed by measuring the dissociation constant using an indirect competition method based on ELISA.

Brief Description:

BiopanningDataSet Comments:

All three peptides share a common feature of an Arg residue flanked by high numbers of Tyr and Trp/Phe residues. The phage display peptide TRWLVYFSRPYLVAT showed an activity 5-fold greater than those of the other two peptides and a dissociation constant of 4.4 nM.

Curator:

Bifang He

Curation Date:

2019-01-29

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