BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3352

Peptides:

Sequences

GWAVFSDIV(2)
GVCSFWESG(2)
TALLWLELT(2)
GVIMLLETI(2)
PFALWQECH(1)
GGFLFSDAL(1)
LTFVFSDHH(1)
GVTVFSDSH(1)
TLSPFMEML(1)
GGSWFMEWE(1)
LDWFFYEIV(1)
GLTTFVDGA(1)
GGAVFVDLG(1)
RNAAFLDQH(1)
EASIFLESH(1)
GFTLFLEDR(1)
GMDLFLDTW(1)
GGTEFLELV(1)
GMTPFADVC(1)
GGFVFADHV(1)
GMLLWEEWA(1)
GGELFRELY(1)
WVTLFHEPH(1)
FLILLSEEH(1)
GGVPLTELL(1)
GGRRLSDDV(1)
GGVVLMDSV(1)
VCQLLMDHH(1)
DLYLLVDDH(1)
VYLQLVDEH(1)
GQMTLVDLH(1)
YFVILLDHH(1)
GVVVLLDNL(1)
AFLLLIEAF(1)
VEVLLLEWV(1)
YVPLLLDFW(1)
GGWFLLDWE(1)
WEMLLWDHH(1)
RAFVLWDTH(1)
GGECLYDAA(1)
RVLTLFDWH(1)
GGITLFELV(1)
VGTGLVRMN(1)
RRAELPGGL(1)
VVTFFAMDH(1)
GGGYYLMLD(1)
MLDFFRFHH(1)
GVWWYKTWC(1)
ELMVYRSHH(1)
PEILWRMVH(1)
GRRGWRRHH(1)
GGGAWRLML(1)
GDCEYEGGK(1)
GVTLFVWAR(1)
GGELFAQCL(1)
GLELFAFAV(1)
GGLEWLWGC(1)
LLRVWVLSV(1)
GGLKWLFTR(1)
GCFMWSGSW(1)
GGGCFSVWS(1)
GLVSFSVYV(1)
GGTVFTSLL(1)
GTEAFGGGG(1)
NVLCFVLGV(1)
GATSYVGCV(1)
GGGMWGATT(1)
AEGLWGSVR(1)
GKGLWGPRR(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 30459762

Target Name:

Cathepsin G (EC:3.4.21.20)

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

CX10C T7 phage display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

An aliquot of the amplified phages were bound to Ni-NTA beads by their His6-tags. Unbound phages were removed by washing ten times in 1.5 ml 1 M NaCl, 0.1% Tween-20 in PBS, pH 7.2, with two subsequent washes with 1.5 ml PBS. The beads were finally resuspended in 1 ml PBS. PBS without protease was used as control. Phages with a random peptide that were susceptible to protease cleavage were released from the Ni-NTA matrix, and the supernatant containing these phages was recovered. To ensure that all of the released phages were recovered the beads were resuspended in 100 μl PBS (pH 7.2) and the supernatant, after mixing and centrifugation, was added to the first supernatant. To ensure that the His6-tags had been hydrolyzed on all phages recovered after protease digestion, 15 μl fresh Ni-NTA agarose beads were added to the combined phage supernatant and the mixture agitated for 15 min followed by centrifugation. A control elution of the phages still bound to the beads, using 100 μl 100 mM imidazole showed that at least 1 × 10^8 phages were attached to the matrix during each selection.

BiopanningDataSet Comments:

Curator:

Bifang He

Curation Date:

2019-01-29

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