BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3349

Peptides:

Sequences
SGQVPWEEPYYVVKKS WEYHYVDLNWTSQHPQ DLPDIIWDFNWETAG

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

5-6

Reference:

PMID: 30128049

Target Name:

Human sural nerve tissue

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

TriCo-16™ phage display peptide library (X16)

Affinity Measurement Method:

Fluorescence

Affinity Measurement Description:

To test the affinity of the selected phage display peptides for binding to human nerves, they were chemically synthesized by solid-phase synthesis and labeled with fluorescein

Brief Description:

Prior to positive selection, phage were counter-selected for high-affinity muscle and fat tissue binding by pre-adsorbing the tissues with the library. For positive selection, phage libraries were mixed directly with human sural nerve tissue.

BiopanningDataSet Comments:

We have now used phage display to identify a novel peptide HNP401 (SGQVPWEEPYYVVKKS) that, when labeled with a fluorophore, selectively binds and highlights human nerves. We show that fluorescently labeled HNP401 can bind to and highlight human sensory and motor nerves such as sural, medial antebrachial cutaneous, laryngeal, ansa cervicalis, great auricular nerve and autonomic nerves like those within and around the prostate gland.

Curator:

Ying Wang

Curation Date:

2018-10-20

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