BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3348

Peptides:

Sequences	Affinity Values
VSPPLTLGQLLS(23/44)	[2.4 ± 0.01]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 23014564

Target Name:

Fibroblast growth factor receptor 3, FGFR-3

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

To identify high-affinity FGFR3-binding clones, the recovered phage clones were examined by enzyme-linked immunosorbent assay (ELISA) for FGFR3 binding with control phage(vcsM13). When optical density (OD) values of phage clones were two times greater than that of phage vcsM13, they will be considered to have high binding affinity for FGFR3. The OD450 value was reproduced from Figure 1A.

Brief Description:

BiopanningDataSet Comments:

We screened a phage library containing random 12-peptide inserts, using fibroblast growth factor receptor-3 (FGFR3) as bait, and obtained 23 positive clones that share identical amino acid sequences (VSPPLTLGQLLS), named as peptide P3. P3 had high binding afﬁnity to the extracellular domain of FGFR3. We found that P3 inhibited the tyrosine kinase activity of FGFR3 and its downstream ERK/MAPK pathway in chondrocytes. P3 also promoted proliferation and chondrogenic differentiation of cultured ATDC5 chondrogenic cells. In addition,P3 improved the growth of bone rudiments from TDII mice in vitro and rescued the lethal phenotype of mice mimicking human TDII in vivo.

Curator:

Ying Wang

Curation Date:

2018-10-20

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