BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3340

Peptides:

Sequences
SGVYKVAYDCQH SGVYQVAYDWQH ILTCFCPPTWRS SGILTCFCPPRWR

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 30153280

Target Name:

Anti-glomerular basement membrane (GBM) autoantibody

Template Name:

Glomerular basement membrane

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

To identify epitopes recognized by the eluted anti-GBM autoantibody, a combinatorial library peptide (12-mers) phage display kit was utilized (Ph.D.™ 12mer Phage Display Library, New England Biolabs, MA) following manufacturer’s protocol for solution phase panning with affinity bead capture. The bound phages were collected by magnetic field and eluted by incubation for 10 minutes with 1ml of glycine elution buffer (0.2M Glycine-HCl, pH2.2, 1mg/ml BSA).

BiopanningDataSet Comments:

Curator:

Zhongjie Tang

Curation Date:

2018-10-20

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