BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3339

Peptides:

Sequences

Affinity Values

CTDQTPYVC(2/11)
CTDMTPGAC(1/11)
CDRTPYRVC(1/11)
CFDATPHVC(1/11)
CTDGTPHAC(1/11)
CDRTPGRPC(1/11)
CTDKTPGFC(1/11)
CSDRTPYMC(1/11)
CTDRTPWAC(1/11)
CDATPPRLC(1/11)

[1.42 ± 0.12]
[2.24 ± 0.14]
[0.14 ± 0.03]
[NA]
[1.41 ± 0.11]
[0.13]
[2.10 ± 0.08]
[0.33]
[1.30 ± 0.13]
[0.95 ± 0.05]

Sequences Features:

Motif:

X-D-X-T-P-X-X

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 29954402

Target Name:

Anti-splicing factor 3b subunit 1(SF3B1) monoclonal antibody, XC24

Template Name:

Splicing factor 3B subunit 1

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-C7C phage display library (CX7C)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Phage ELISA of selected epitopes against XC24 autoantibody were performed. The OD450 values were shown and reproduced from Figure 3b.

Brief Description:

BiopanningDataSet Comments:

The specific peptide epitopes against XC24 were selected and, among them, XC24p11 cyclic peptide (CDATPPRLC) was used as an epitope of anti-SF3B1 autoantibody ELISA. With this epitope, we could effectively distinguish between serum samples from hepatocellular carcinoma (HCC) patients (n = 102) and healthy subjects (n = 85) with 73.53% sensitivity and 91.76% specificity (AUC = 0.8731). Moreover, the simultaneous detection of anti-XC24p11 epitope autoantibody and AFP enhanced the efficiency of HCC diagnosis with 87.25% sensitivity and 90.59% specificity (AUC = 0.9081). ELISA using XC24p11 peptide epitope that reacts against anti-SF3B1 autoantibody can be used as a novel test to enhance the diagnostic efficiency of HCC.

Curator:

Lixu Jiang

Curation Date:

2018-10-20

First |

Prev |

3339

| Next

| Last