BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3325

Peptides:

Sequences

CRHSQMTVTSRL(15,780)
DWSSWVYRDPQT(14,310)
SHCLARQTMLQV(9,570)
DRWVARDPASIF(7,550)
SQDIRTWNGTRS(2,719)
VEPPNSQHSGPC(2,467)
VHWDFRQWWQPS(2,437)
AYYPFVKGRPLP(1,435)
MHPNAGHGSLMR(1,428)
ASSRRPSVGKGF(1,379)
VTTMSGEISQAR(962)
GFPTRFEALSSN(828)
TGSNSPMWDTEV(722)
HVRLTGWQPTWA(306)
QFDYMRPANDTH(298)
SPCTSFPRCVEL(293)
QLATLHKLSGPT(278)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Next generation sequencing

Rounds of Panning:

Reference:

PMID: 29875202

Target Name:

CD63 protein fragment containing the second extracellular loop (CD63 LEL)

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Phages (1e12 plaque-forming units) were incubated with CD63 LEL purified protein for 2 hours, and unbound phages were removed by washing with PBS solution for three times. The bound phages were recovered with glycine-HCl buffer (pH 2.2), followed by neutralization with tris-HCl buffer (pH 8.8). Recovered phages were titered and amplified for subsequent cycles. This process was repeated twice, and the recovered phage clones’ sequences were identified by high-throughput sequencing (Genewiz).

BiopanningDataSet Comments:

One peptide in particular, CP05, enabled direct painting of exosomes with different moieties irrespective of the origin of exosomes.

Curator:

Lixu Jiang

Curation Date:

2018-07-23

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