BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3317

Peptides:

Sequences	Affinity Values
IMPHKHRRKLRL(1) MLTISQRQLSIS(1) RNRTKKQITTQR(1) RMKMLMMLMRRK(11)	[0.50 ± 0.02] [0.48] [0.56 ± 0.01] [0.52]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 29517966

Target Name:

Anti-RhD monoclonal antibody

Template Name:

Blood group Rh(D) polypeptide

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Plates were coated with monoclonal anti-D at 4 °C overnight. Phage clones were added to the antibody-coated wells and incubated for 1 h at room temperature. The primary phage library was used as the negative control The optical density (OD) at 450 nm was subsequently recorded using a microplate reader.

Brief Description:

In the second and third rounds of biopanning, 75 μg/mL and 50 μg/mL anti-D were incubated with 1e11 amplified phages selected in the first biopanning round for enrichment of high affinity binding. In addition, the bound phages were merely titered but not amplified in the third round.

BiopanningDataSet Comments:

The secondary structure of the synthesised peptide was predicted, based on its amino acid sequence, using PSIPRED (Psi-blast based secondary structure prediction) methods14. The I-TASSER server was employed to predict the three-dimensional structure15. Rasmol, version 2.7.5 (www.RasMol.org) was applied for the visualisation.

Curator:

Pengcheng Yao

Curation Date:

2018-04-19

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