BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3316

Peptides:

Sequences

Affinity Values

YSLRSDY(5)
YSLRQDW(3)
YSLRTDW(3)
YSLRQER(3)
YSLRADR(2)
ALSSLRN(2)
HSIRYDF(2)
HSIRVDW(2)
KVWIVPS(2)
KVWLLHS(2)
DWIFPAF(1)

[0.59±0.02]
[0.37±0.01]
[0.48±0.01]
[0.50±0.01]
[0.39±0.01]
[0.55±0.02]
[0.62±0.01]
[0.35±0.01]
[0.50±0.01]
[0.49±0.02]
[0.43±0.02]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sangon sequencing

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 29301517

Target Name:

Glycoprotein G

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

ELISA plates were coated with the G1 protein and the purified pET32a(+) vector control protein coated with the same concentration. The absorbance of the reaction was determined at 450 nm with an ELISA microplate reader.

Brief Description:

BiopanningDataSet Comments:

27 specific peptide ligands displaying 11 different amino acid sequences were obtained, and the HSIRYDF and YSLRSDY clone had a higher affinity to G1 protein than the other clones.

Curator:

Pengcheng Wu

Curation Date:

2018-04-19

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