BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3275

Peptides:

Sequences	Affinity Values
CSAYDRPLC(12/60) CWSNKGYDC(2/60)	[0.74 ± 0.01] [0.79 ± 0.01]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 29156833

Target Name:

Peptide KEQWFGNRWHEGYR

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-C7C phage display library (CX7C)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Eleven phage clones with the highest selection frequency and unique sequences were chosen for analysis of their ability to bind the v6 region peptide using phage ELISA. The optical densities were measured with a μQuant Universal Microplate Spectrophotometer (Bio-Tek Instruments, Winooski, VT) at 490 nm. The data shown were reproduced from Figure 1C.

Brief Description:

Libraries were selected against a v6 region peptide derivative, KEQWFGNRWHEGYR, which encompassed a 14 amino acid sequence of v6 domain of human CD44v6 protein. After three cycles of affinity selection, sixty individual phage clones from the elution pool of the M13C7C library were randomly chosen for DNA sequencing.

BiopanningDataSet Comments:

Curator:

Juanjuan Kang

Curation Date:

2018-01-19

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