BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3229

Peptides:

Sequences	Affinity Values
HCSSAVGSWTWENGKWTWKGIIRLEQ CSSPIQGSWTWENGKWTWKGIIRLEQ HSCSSPIQGSWTWENGKWTWKGIIRLEQQP	[65] [16] [3]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 22271427

Target Name:

Fibronectin extradomain B, EDB

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Aptide pIGT2-based phage display library (X6GSWTWENGKWTWKGX6)

Affinity Measurement Method:

Surface plasmon resonance (SPR)

Affinity Measurement Description:

Kinetic binding data (Kd, nM) for selected aptides, as determined by affinity measurements using BIAcore chips adapted for surface plasmon resonance.

Brief Description:

To immobilize the target protein (biotin-fibronectin extradomain B), streptavidin was immobilized directly on 96-well plates overnight at 4°C, and then the target protein was immobilized on the plate-bound streptavidin.

BiopanningDataSet Comments:

Curator:

Bifang He

Curation Date:

2017-10-24

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