BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3214

Peptides:

Sequences

HSVKPVVNLILR(34)
HSIRLHTYPHMK(19)
YSLRADSRWMPS(18)
HSLRPEWRMPGP(15)
HSIRTYWQSAQP(10)
HSLKPSWLLLGY(2)
HSLKPSLKQLAI(2)
HSLREDWTLRMQ(2)
APPGNWRNYLMP(2)
TMGFTAPRFPHY(2)
TMHSLRPEWRMP(1)
HSVKPDWAQMLR(1)
HSVKHDFRLLTK(1)
HSIRSSHLHMFT(1)
SGHQLLLNKMPN(1)
APRLPQSLLPQL(1)
SHALPLTWSTAA(1)
APPMSRQSFDGV(1)
NFMESLPRLGMH(1)
LLADTTHHRPWT(1)
MEGQYKSNLLFT(1)
NTELTSYGPPPA(1)

Sequences Features:

Motif:

H-S-[ILV]-[KR],[KR]-X-P, [KR]-X-X-P

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning, competitive panning)

Sequencing Platform:

Sequencing Method:

Sanger

Rounds of Panning:

Reference:

PMID: 28415614

Target Name:

Gap junction alpha-5 protein

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

A phage library was first pre-cleared in an uncoated well, and then presented to the bait protein. Low-affinity binders were first eluted using a solution of free bait protein in TBS.

BiopanningDataSet Comments:

One of the retrieved peptides (HSLRPEWRMPGP) showed a 58.3% homology with amino acids 5-to-16 of IκBα, a member of the protein complex inhibiting NFκB activation. Binding of IκBα (peptide) and Cx40 was confirmed by crosslinking and en face proximity ligation assay on carotid arteries.

Curator:

Yewei Fang

Curation Date:

2017-07-22

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