BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3180

Peptides:

Sequences
SCNVAGGTCGLT YPSSEQLLAWWG VAGPQITKYNVP ESSPATKFVRPS VCLYVNIECSQQ LGPQRQENSHPF GLPIWPQHLTNT RMDPDQPRARVA GFGGLVDDVRGG INIPDEQSSNHL QSDPWLVSRWFA ALVNQADGDSTQ SLYGKKSPMGYP YSDEGTPRSGGL WPTDHQMLRIPM

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 26161996

Target Name:

IL-4-polarized M2 macrophage

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Subtractive phage display using murine IL-4- polarized M2 ("anti-inflammatory") macrophage for positive selection and IFN-γ- and LPS-polarized M1 ("pro-inflammatory") macrophage for negative selection was completed in biological duplicates with one initial enrichment selection followed by three rounds of paired negative/positive selection.

BiopanningDataSet Comments:

From the last round of each duplicate phage display experiment, 20 phage plaques were randomly selected for Sanger sequencing (replicate 1).

Curator:

Lixu Jiang

Curation Date:

2017-05-09

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