BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3179

Peptides:

Sequences

WPTDHQMLRIPM(32.14%)
TYPSTQWFFAKF(9.51%)
SFPDVQWWRNQY(5.16%)
WPWDPLRISDWL(5.08%)
TLPPEAWWRTMY(4.42%)
QSDPWLVSRWFA(3.59%)
YPSSEQLLAWWG(3.52%)
RLPTSMELLAAF(3.21%)
GLPSSAELERLW(2.47%)
ELPSVEQLWDFF(2.44%)
TWVSDLDMWLGA(1.89%)
QDPWKVKNWINQ(1.78%)
FFPSEQVLIAAL(1.64%)
SDSWVRGLNYWY(1.27%)
FDPLNIRHWIWG(1.20%)
WDPWQIDRWVLL(1.19%)
LPWPSDQIILMW(1.17%)
DPWFINEWIRIP(0.88%)
LPSSAELLWALR(0.81%)
SYWVPDIVWAGL(0.74%)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Illumina MiSeq system

Sequencing Method:

Next-generation sequencing

Rounds of Panning:

Reference:

PMID: 26161996

Target Name:

IL-4-polarized M2 macrophage

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Subtractive phage display using murine IL-4- polarized M2 ("anti-inflammatory") macrophage for positive selection and IFN-γ- and LPS-polarized M1 ("pro-inflammatory") macrophage for negative selection was completed in biological duplicates with one initial enrichment selection followed by three rounds of paired negative/positive selection.

BiopanningDataSet Comments:

Barcoded phage library amplicons were sequenced by Illumina, and the top 20 most abundant sequences from the last rounds are shown (replicate 2).

Curator:

Lixu Jiang

Curation Date:

2017-05-09

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