BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3166

Peptides:

Sequences

RIRSEEL(24)
TVVVAAG(17)
VQLSIIV(15)
PRTTIMG(15)
KFVVAFC(14)
LAPREGA(13)
LMTARWC(13)
FWALATW(13)
YCVGDGC(13)
WVNAATC(12)
SRGVGVG(12)
RRRPGAV(12)
QLVRGRW(12)
SVFIMLR(11)
VFMGGCR(11)
TSEGALR(11)
SSRSSGG(11)
GRLSADG(11)
RRAVRFM(11)
GLCAEAC(10)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Sequencing Platform:

Illumina HiSeq 2000 and/or Genome Analyzer Iix

Sequencing Method:

Next-generation sequencing

Rounds of Panning:

Reference:

PMID: 22870226

Target Name:

Not determined.

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

pVIII fth1-dp-based X7 phage display library (X7)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

For the analysis of the naive libraries, four samples of 4.0e8 phages each were amplified individually by the PCR reactions. The amplified PCR products were validated for size (152 bp) by running in 2% agarose gel. All 4 PCR cleaned products were united and their concentration was measured. The sample was dried by speed vac and sent for Illumina sequencing.

BiopanningDataSet Comments:

The 20 top most frequent peptides for each capture are given.

Curator:

Lixu Jiang

Curation Date:

2017-05-09

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