BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3129

Peptides:

Sequences

YEQHKLPSSWPF(12)
YQTKLQHVWPFS(4)
SPYQTRLPVVWP(3)
LSQSKLASPWPL(1)
QISQLRLPNNWP(1)
FQTRLLHTWPSP(1)
EHQVRLSSQWPL(1)
TEQMKLLYHWPT(1)
QYKIPPLWPMLK(1)
YQMKSPTPVWPF(1)
HHWFKSLTTKHL(1)
YQMKLPLILPPT(1)
DFQNKLSSVTLR(1)
ISQQPLAPVWPP(1)
DAQQLYLSNWRS(1)
NQHPPMAHLSAQ(1)
YVHHSQLPDASL(1)
HAKSQLNPPDIK(1)
SAHQVPNAVIKS(1)
GQGVPDLHPPLL(1)
LPNPQQYTTPSS(1)
RYPLESPYWQQT(1)
TSPLNIHNGQKL(1)
DSTNMETQTLPL(1)
DLNTNRTQMVLH(1)
HESFWYLPHQSY(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger Sequencing

Rounds of Panning:

Reference:

PMID: 18959752

Target Name:

Protein-glutamine gamma-glutamyltransferase K

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

BiopanningDataSet Comments:

The peptides selected as glutamine donor substrate exhibited a marked tendency in primary structure, conforming to the sequence: QxK⁄RψxxxWP (where x and ψ represent non-conserved and hydrophobic amino acids, respectively). Using glutathione S-transferase (GST) fusion proteins of the selected peptides, we identified several sequences as preferred substrates and confirmed that they were isozyme-specific. We generated GST-fused alanine mutants of the most reactive sequence (K5, YEQHKLPSSWPF) to determine the residues that were critical for reactivity. Even in peptide form, K5 appeared to have high and specific reactivity as substrate. In situ analysis of mouse skin sections using fluorescence-conjugated K5 peptide resulted in detection of TGase 1 activity with high sensitivity, but no signal was detected in a TGase 1-null mouse.

Curator:

Bifang He

Curation Date:

2017-04-11

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