BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3074

Peptides:

Sequences

Affinity Values

SRNLSYAEYIQI
GNYSEYIVGKLV
SSYANYIQFRNT
SSYTAYIMARGQ
NSMSEYINYILT
VDYSTYISRLTS
NFMNYAEYVQKK
VDYSTYISRLTS
TVHSYEEYTARR
VSPYAEYWLSQM
WDYNLYIKYVAR
VDYATYISRLTS

[1.53]
[1.19]
[1.02]
[1.19]
[1.25]
[1.19]
[1.45]
[1.22]
[1.01]
[1.39]
[1.12]
[1.40]

Sequences Features:

Motif:

Y-A-E-Y-I

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 27834908

Target Name:

Anti-E protein monoclonal antibody (mAb) 1A5

Template Name:

E protein

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

The OD450nm value was measured. The data shown were reproduced from Figure 1.

Brief Description:

BiopanningDataSet Comments:

One minimal epitope was mapped to 87-YAEYI-91 by using phage display and mutagenesis. Duck Tembusu virus (DTMUV) positive duck sera reacted with the epitope, thus indicating the importance of the minimal amino acids of the epitopes for antibody-epitope binding. The performance of the dot blotting assay with the corresponding positive sera indicated that YAEYI was DTMUV type-specific. The structure model of the E protein revealed that YAEYI was located on domain (D) II, which confirmed that DII might contain a type-specific non-neutralizing epitope. The YAEYI epitope-based antigen demonstrated its diagnostic potential by reacting with high specificity to serum samples obtained from DTMUV-infected ducks. Based on these observations, a YAEYI-based serological test could be used for DTMUV surveillance and could differentiate DTMUV infections from JEV or WNV infections.

Curator:

Curation Date:

2017-01-13

First |

Prev |

3074

| Next

| Last