BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3073

Peptides:

Sequences

Affinity Values

ELNLPWQRNALV
SAENDLTLPWTT
MANAEIDLPWTK
HPHDLNDLTSPF
EFWTALSDPWYF
AHLHDPFTTLSP
LDFHDLNRPFNN
THDPLDSPWNFS
FNDLDLPFGKRA
SYDLDLPWIARK
SFLELDPPWTTN
QHSFLDLPWHLT
HPHDLNLPTSPF
HPHDLNLPTSPF
MANADLNLPWTK
TSHSWDLNLPSG

[1.47]
[1.14]
[1.23]
[1.13]
[1.29]
[1.00]
[1.29]
[0.99]
[1.11]
[1.08]
[1.16]
[1.08]
[1.33]
[1.22]
[1.37]
[1.43]

Sequences Features:

Motif:

D-L-[DN]-L-P-W-T

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 27834908

Target Name:

Anti-E protein monoclonal antibody (mAb) 1F3

Template Name:

E protein

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

The OD450nm value was measured. The data shown were reproduced from Figure 1.

Brief Description:

BiopanningDataSet Comments:

One minimal epitope was mapped to 221-LD/NLPW-225 and by using phage display and mutagenesis. Duck Tembusu virus (DTMUV) positive duck sera reacted with the epitope, thus indicating the importance of the minimal amino acids of the epitope for antibody-epitope binding. The performance of the dot blotting assay with the corresponding positive sera indicated that 221-LD/NLPW-225 was a cross-reactive epitope for West Nile virus (WNV), dengue virus (DENV), and Japanese encephalitis virus (JEV) and corresponded to conserved and variable amino acid sequences among these strains. The structure model of the E protein revealed that LD/NLPW was located on domain (D) II, which confirmed that DII might contain a type-specific non-neutralizing epitope.

Curator:

Curation Date:

2017-01-12

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