BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3025

Peptides:

Sequences	Affinity Values
LAKPHTENHY(4/32) QNPIQKIIY(1/32) LAKPHTENHLLT(1/32)	[1.02 ± 0.11] [0.81] [1.16]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 27491899

Target Name:

The envelope protein Ampho4070A

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

The binding activities of the positive phage clones to the envelope protein Ampho4070A expressed by retrovirus like particles were measured by phage ELISA. The OD450 values were measured and data shown were reproduced from the Fig. 1b in the reference.

Brief Description:

In order to eliminate unspecific binders several depletion steps were incorporated in the panning campaigns using the VLP null particles as well as non-coated plates.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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