BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2971

Peptides:

Sequences	Affinity Values
RRPRMLT QLRRQRR HSRRMRK RSRRIRL	[1.40 ± 0.23] [NT] [NT] [5.37 ± 0.49]

Sequences Features:

Motif:

R-R-x-R

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 17699853

Target Name:

Neuropilin-1

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-C7C phage display library (CX7C)

Affinity Measurement Method:

Surface plasmon resonance (SPR)

Affinity Measurement Description:

Surface plasmon resonance was used to measure the real-time association and dissociation of the binding of RRXR-containing peptides to NRP1. The average dissociation constants (KD) for the binding of DG1 and DG2 to NRP1 were 1.40 ± 0.23 and 5.37 ± 0.49 umol/L, respectively.

Brief Description:

BiopanningDataSet Comments:

Two potent synthetic anti-NRP1 peptides, CRRPRMLTC and CRSRRIRLC, which block NRP1 signaling pathways and suppress tumorigenesis, cancer invasion, and angiogenesis, were identified.

Curator:

Curation Date:

0000-00-00

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