BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2970

Peptides:

Sequences
LSLTSTL LPTPALR ASGLLSL SLTSTLY TLLVQGS LPLNSSA LSLPLNS SSSLTLK VTSALSR SALSRDG RLSVPPL GLLSLTS GSPSTPA LSLSSIT SLTLKVT KVLSLPL SLPLNSS LSVPPLV

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Illumina HiSeq 2000

Sequencing Method:

Next generation sequencing

Rounds of Panning:

Reference:

PMID: 27143691

Target Name:

Human KRAS-mutant colorectal cancer cell lines and tissues

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

The ex-vivo screenings were run on pooled specimens from different animals (n=3), with a standard procedure that involved two successive selection rounds (R1, R2) and two alternative controls (C1, pseudo-metastasis from WT cells; C2, non-tumoral liver). The in vitro screenings were performed with the same standard procedure (P1) or with an enrichment protocol (P2) in which several rounds were iterated until increased phage binding to KRAS mutant vs WT cells was observed. Each KRAS mutational status (G12D, G12V, G13V) was independently screened in 4 biological replicates, i.e. 2 cell lines (SW-48, LIM1215) × 2 clones, and in 2 experimental conditions (ex-vivo and in vitro) for a total of 24 phage display screening experiments. Each of them consisted of 5 output samples (3 mutant KRAS + 2 controls), giving a total of 120 samples.

BiopanningDataSet Comments:

This subset of peptides has been derived from a larger, deep-seq analysis. These peptides are identical to regions of the human BCAM protein and are potential ligands for LAMA5.

Curator:

Curation Date:

0000-00-00

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