BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2951

Peptides:

Sequences
AELAKLPLF GAISFDHVHP

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 26543513

Target Name:

Colorectal cancer cell(HCT-8)

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Negative selection using magnetic beads to capture M13 phage display peptides that were not of interest was first performed such that peptides binding on non-target cells (control cells) could be removed. Then, the supernatant was collected to perform the subsequent positive selection. As mentioned previously, the target cells surfaced-coated with magnetic beads were incubated with the supernatant to perform the positive selection.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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