BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2950

Peptides:

Sequences	Affinity Values
RDGSIAMHSMIP(1) QALLEGNAKGGN(1) GGTKTHVDFSLK(1)	[0.36 ± 0.01][0.27 ± 0.02] [NT][NT] [NT][NT]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 26543535

Target Name:

Maltase-glucoamylase (MGAM)

Template Name:

Anti-MGAM polyclonal antibody

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

The binding activities of the positive phage clones to the maltase-glucoamylase were measured by phage ELISA and competitive phage ELISA. The A450 values were measured and data shown were reproduced from the Fig. 1a in the reference. In the competitive phage ELISA, a competitive inhibitor (0.5 mmol/L acarbose and 0.5 mmol/L voglibose) was added. M13 phage vector showing no peptide was used as control, and the corresponding affinity values were 0.17 ± 0.01 and 0.08 ± 0.01 respectively. NT means not test.

Brief Description:

In the second and third round of selection, an aliquot of amplified eluate from a previous round of biopanning was added to anti-MGAM antibody-coated beads. After 30 min of gentle shaking at room temperature, the supernatant was transferred to the enzyme-coated beads and was incubated for 60 min.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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