BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2867

Peptides:

Sequences
QTTHRNWA (8) HTSHRNWD (4) FPHPWNPP (3) YPHPWNPT (3) PRYLPWFP (2)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 23394544

Target Name:

Anti-aflatoxin monoclonal antibody 1C11

Template Name:

Aflatoxin B1

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

CX8C M13 phage display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

To eliminate nonspecific binding of phage with BSA, phages were firstly incubated with plate coated with BSA-PBS. After incubation, the supernatant containing unbound phages was incubated with the the plate containing 1C11 MAb. To elute the bound phage, 100 μL AFB1 (100 ng/mL in 10% methanol, competitive elution) was added to each well with shaking for 30 min to compete the binding phage from the coating antibody.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

First |

Prev |

2867

| Next

| Last