BDB - Library Details

BiopanningDataSet
Target
Template
Library
Structure

Library

Library Name: CX8C M13 phage display library

Library ID:

446

Library Length:

Library Complexity:

Library Titer:

3.4e13

Library From:

Library Randomness:

Completely random

Library Scheme:

NNK

Library Topology:

Circular

Comments:

The library was made by ligating a synthetic 42-bp fragment into the M13KE vector and transfecting E. coli cells with the ligation product by electroporation. Within the fragment was the degenerate coding sequence 5′-GCTTGT (NNK)8 TGCGGTGGAGGT-3′, where N stands for an equimolar mixture of G, A, T, and C while K is an equimolar mixture of G and T. The single strand degenerate oligonucleotide is converted to double strand by polymerase chain reaction (PCR) using the extension primer 5′-CATGCCCGGGTACCTTTCTATTCTC-3′ (New England Biolabs Inc., Ipswich, MA).

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