BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2864

Peptides:

Sequences	Affinity Values
AAAPLAQPHMWA(4) SSTTTSDKYLSA(2) SNLHDNNTEKNV(1)	[11.50 ± 0.56, 13.7] [9.31 ± 1.46, 10.8] [8.06, 9.3]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 25826583

Target Name:

Tumor necrosis factor ligand superfamily member 13

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Phage ELISA was used for identification of specific sAPRIL-binding phage. Clone 21 (sequence not shown) was used as a positive control. The fold change of the optical density was normalized to the positive control. Clones with an A450 that was at least 6 fold higher than the positive control were considered positive for sAPRIL binding. Besides, three binding peptides were synthesized and their binding affinity with sAPRIL was determined and compared with an unrelated polypeptide (HWDPFSLSAYFP) as a negative control using peptide ELISA. Data in the first column of affinity values were results for phage ELISA and reproduced from Fig. 1A in the reference. Data in the second column of affinity values were results for peptide ELISA and reproduced from Fig. 1B in the reference.

Brief Description:

BiopanningDataSet Comments:

The sAPRIL-BP1 (AAAPLAQPHMWA) had the highest binding affinity and was subsequently used in vitro to assess whether it was able to inhibit sAPRIL binding in the fixed human colorectal cancer cell line LOVO cells. sAPRIL-BP1 showed a dose-dependent inhibitory effect on sAPRIL binding to the LOVO cells. In vivo in a mouse colorectal challenge model, the sAPRIL-BP1 reduced the growth of tumor xenografts in nude mice by inhibiting proliferation and inducing apoptosis intratumorally. Moreover, in an in vivo metastasis model, sAPRIL-BP1 reduced liver metastasis of colorectal cancer cells. It might be a candidate for treating colorectal cancers that express high levels of APRIL.

Curator:

Curation Date:

0000-00-00

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