BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2863

Peptides:

Sequences	Affinity Values
LPLGPHT TDHSMPP SSPPKSP	[0.066 ± 0.002] [0.155 ± 0.002] [0.131 ± 0.002]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 25515813

Target Name:

Fibroblast growth factor 23(180-205)

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Affinities of the positive phage clones to FGF23 were measured by phage ELISA. The OD values were measured at 450 nm with a reference wavelength of 655 nm and data shown were reproduced from Fig. 1 in the reference. The affinity value of control phage vcsM13 to FGF-23 is 0.002. The affinity value was presented as mean ± standard deviation.

Brief Description:

BiopanningDataSet Comments:

After four rounds of panning, three positive clones with higher affinity and specificity for FGF23 were selected from 20 clones detected and further subjected to sequencing.

Curator:

Curation Date:

0000-00-00

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