BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2672

Peptides:

Sequences
SSTSSVGRAFDVWRAAVLYSTHAVPPEQSSR SSLGLNSGDRTGWRAAVDQLLRLNKNKFDSR SSAVTDPATRSTKWAAAVADIIRSKNMQKSR

Sequences Features:

Motif:

WRAAV

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 8635738

Target Name:

Calmodulin, CaM

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

R26 M13 phage display library (SRX12(A/S/P/T)A(A/D/E/G/V)X12SR)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

CaM (0.2 μg/well) was immobilized to 96-well polystyrene microtiter plates by non-specific absorption in 0.1 M NaHCO3 (pH 8.5). Biotinylated CaM (0.2 μg/well) was immobilized using streptavidin (0.5 μg/well) absorbed as above. For libraries screening, e11 phage particles were incubated with immobilized CaM, non-binding phage washed away, binding phage recovered by acid elution and amplified in E. coll. Three rounds of screening were performed.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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