BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2644

Peptides:

Sequences
SLDELDWDSM(4) RHEDGDWPRV(1) LLDDGFWPRV(1) CGVDGRWPRW(1) SLISDEWPRW(1) DGEWPREGWS(1) EDLEGEWPMR(1)

Sequences Features:

Motif:

[DE]-G-[DE]-W-P-R

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

3/4

Reference:

PMID: 9050886

Target Name:

Anti-dsDNA monoclonal antibody 52b3

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

L100 phage display library (X10)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

For the first round of selection, 50 μl of streptavidin-coated magnetic beads were incubated with 100 μl of 5 μM biotinylated protein G for 1 hr at room temperature with agitation. In subsequent rounds of selection, 10 μl of streptavidin beads and 0.05 μM of biotinylated protein G were used. The streptavidin bead/biotinylated protein G complex was combined with the phage/antibody mixture and incubated.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

First |

Prev |

2644

| Next

| Last