BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2530

Peptides:

Sequences
VHHSCTKLTHCCQNWH(0.52) GGVSCMQTSPVCENNL(0.24)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 18723274

Target Name:

Tumor-associated glycoprotein (TAG-72)

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

f88-Cys6 phage display library (X4CX6CX4)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

To begin one selection cycle, the three coated wells of one plate were washed and then blocked with 0.1 M sodium bicarbonate buffer containing 0.5% BSA. The phage library, containing about 2e8 phages in 400 μl of TBS/Tween and 0.1% BSA, was added first to the well coated with the TAG-72 free eluant from the affinity column. After incubation for 20 min the library was transferred and incubated for 30 min in the BSA coated well. Thereafter, the library was transferred to the final well containing the purified TAG-72 and incubated with gentle mixing for 1 hr at room temperature.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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