BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2461

Peptides:

Sequences	Affinity Values
CHLGYPGRC CHYSYPGVC CHLNYPGYC CHLRYPGEC CYKGYPGYC	[0.656 ± 0.032] [0.811 ± 0.045] [0.846 ± 0.152] [1.087 ± 0.105] [0.293 ± 0.009]

Sequences Features:

Motif:

YPG

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 24497417

Target Name:

Anti-Claudin 18.2 monoclonal antibody IMAB362

Template Name:

Claudin 18.2

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-C7C phage display library (CX7C)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Binding of picked clones to IMAB362 was detected by Phage ELISA. Absorbance at 450 nm was determined. Data shown were reproduced from the graph and expressed as the mean of two experiments ± S.D.

Brief Description:

BiopanningDataSet Comments:

Three of the peptide sequences (CHLGYPGRC, CHYSYPGVC and CHLNYPGYC) were selected for further binding, SAR analysis, and optimization via peptide microarrays.

Curator:

Curation Date:

0000-00-00

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