BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2400

Peptides:

Sequences
YMDSGCAQGWEEYCMWECS

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 11075354

Target Name:

Bovine insulin-Like growth factor-binding protein 2, bIGFbp-2

Template Name:

Insulin-like growth factor I, IGF-I

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

X5CX8CX5 phage display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

BlGFbp-2 was coated directly onto immunosorbant plates. The library was subjected to three rounds of selection, using 100 mM HC1 elution, followed by neutralization. Phage from round 1 were amplified in E. coli before use in round 2. The eluted phage from round 2 were not amplified before use in round 3.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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