BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2259

Peptides:

Sequences	Affinity Values
HHKTWHPPVMHL(8) SQWHPRSASYPM(2)	[1.415 ± 0.061] [1.355 ± 0.035]

Sequences Features:

Motif:

WHP

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 23664850

Target Name:

Spike glycoprotein

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Ten selected phages were incubated with the SARS-CoV S1 protein in ELISA plates to determine the specific binding activities for the protein. Three independent experiments were repeated for each individual. The OD492 value of tested individual phage was determined, reproduced from the graph and expressed as mean ± SD. The absorbances of phages with the same sequence were calculated as mean ± SD. The value of the negative control was 0.377 ± 0.071.

Brief Description:

BiopanningDataSet Comments:

The binding activities of 10 selected phages were assayed using ELISA. The results revealed that they had a specific binding activity to the SARS-CoV S1 protein.

Curator:

Curation Date:

0000-00-00

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