BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1905

Peptides:

Sequences
FHKHKSPALSPV(19) FHKPFFPKGSAR(13)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 22138765

Target Name:

Tenascin, TN

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Most solid tumors express the large isoform of TNC which contains EGF domain, fibrinogen glob (fbg) and fibronectin III (FN III) domains with the inclusion of alternatively spliced exons in the middle. Due to the complexity and sizes of the tumor specific TNC protein, the authors utilized two different versions of TNC proteins and performed two independent selections. One is the tumor-specific large isoform of TNC (Full-length TNC) expressed in eukaryotic cells and the other is the alternatively spliced domain (A1-D) of TNC expressed in bacteria.

BiopanningDataSet Comments:

Of the 35 clones derived from two independent selections, 19 had the same sequences (designated peptide #1), and 13 others were also identical (designated peptide #2), while the remainder had similar sequences. The selected peptide #1 specifically recognized tenascin C protein in xenograft mouse tissue. The authors also observed exclusive staining of tenascin C by the selected peptide in tumor patient tissues. Moreover, the peptide reduced tenascin C-induced cell rounding and migration.

Curator:

Curation Date:

0000-00-00

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