BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1839

Peptides:

Sequences
CVKWRGVVVC

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 21821708

Target Name:

Membrane primary amine oxidase

Template Name:

Sialic acid-binding Ig-like lectin 9 (Siglec-9)

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

CX8C phage display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

BiopanningDataSet Comments:

After 4 rounds of panning using recombinant VAP-1 as a bait, we obtained a 400-fold enrichment of phages bound to VAP-1 compared with the control (BSA-coated wells). Of the 23 randomly selected phages sequenced, 19 gave a sequence. Database searches with the sequence derived from the phage clones revealed similarities to the amino acid sequence of Siglec-9 (residues 289-295). In the binding assays, the phage peptide bound selectively to recombinant VAP-1. The binding between Siglec-9 and VAP-1 was confirmed by in vitro and ex vivo adhesion assays. The interaction sites between VAP-1 and Siglec-9 were identified by molecular modeling and confirmed by further binding assays with mutated proteins.

Curator:

Curation Date:

0000-00-00

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